Part:BBa_K2919000:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2919000

This part was used in an attempt to make a toxin-antitoxin saftey system that would limit horizontal gene transfer. The part was used with a cumate inducible, CuO, promoter to prevent the toxin to kill the cell immediately. When induced with sufficient cumate the toxin worked to prevent cell growth in E. coli.

This graph show that the toxin, ghoT, works by showing that the E. coli with the toxin insert when induced by cumate had lower OD600s than the E. coli without the toxin insert.

User Reviews

UNIQce7dcbc74be19ed5-partinfo-00000000-QINU UNIQce7dcbc74be19ed5-partinfo-00000001-QINU